Device

Part:BBa_K4611009:Design

Designed by: Tuo Wang   Group: iGEM23_CJUH-JLU-China   (2023-10-05)


His-SUMO-LwaCas13a with lacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1082
    Illegal EcoRI site found at 4383
    Illegal PstI site found at 4404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1082
    Illegal EcoRI site found at 4383
    Illegal PstI site found at 4404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1082
    Illegal EcoRI site found at 4383
    Illegal BglII site found at 1363
    Illegal BglII site found at 2839
    Illegal BglII site found at 3049
    Illegal BglII site found at 3316
    Illegal BglII site found at 4081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1082
    Illegal EcoRI site found at 4383
    Illegal PstI site found at 4404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1082
    Illegal EcoRI site found at 4383
    Illegal PstI site found at 4404
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Assembled by CJUH-JLU-China. Basic parts are provided by pc013-Twinstrep-SUMO-huLwCas13a(addgene).


References